2A6B
Crystal structure of a putative transcriptional regulator of the tena family (spr0628) from streptococcus pneumoniae r6 at 1.70 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-01 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 119.908, 119.908, 47.105 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.980 - 1.700 |
Rwork | 0.155 |
R-free | 0.17500 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.304 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.980 | 29.980 | 1.740 |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.089 | 0.068 | 0.513 |
Number of reflections | 38398 | ||
<I/σ(I)> | 4.4 | 7.2 | 1.3 |
Completeness [%] | 100.0 | ||
Redundancy | 9.4 | 7.3 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 273 | o.2M LiCl, 20.0% PEG-3350, No Buffer pH 6.7, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 273K | |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 273 | o.2M LiCl, 20.0% PEG-3350, No Buffer pH 6.7, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 273K |