2A5G
Cholera toxin A1 subunit bound to ARF6(Q67L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.067, 44.484, 56.045 |
| Unit cell angles | 107.38, 101.72, 102.94 |
Refinement procedure
| Resolution | 18.520 - 2.660 |
| R-factor | 0.20217 |
| Rwork | 0.199 |
| R-free | 0.26519 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CTA1:ARF6-GTP |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.717 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Number of reflections | 8923 | |
| Completeness [%] | 75.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 2000mme, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
