2A3Z
Ternary complex of the WH2 domain of WASP with Actin-DNAse I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.270, 41.798, 119.418 |
| Unit cell angles | 90.00, 108.68, 90.00 |
Refinement procedure
| Resolution | 40.100 - 2.078 |
| R-factor | 0.15907 |
| Rwork | 0.153 |
| R-free | 0.21030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1atn |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.847 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.100 | 2.140 |
| High resolution limit [Å] | 2.078 | 2.078 |
| Rmerge | 0.079 | 0.379 |
| Number of reflections | 42018 | |
| <I/σ(I)> | 20.2 | 3.4 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 6.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | sodium formate, PEG2000 MME, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
