2A3M
Structure of Desulfovibrio desulfuricans G20 tetraheme cytochrome (oxidized form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.670, 43.876, 57.729 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.960 - 1.500 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.19500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cth |
RMSD bond length | 0.012 |
RMSD bond angle | 1.218 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
Rmerge | 0.060 | 0.036 | 0.238 |
Number of reflections | 18098 | ||
Completeness [%] | 98.0 | ||
Redundancy | 6.4 | 6.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 20% PEG 4000, 5% ethylene glycol, 0.2M ZnCl2, 0.1 M imidazole, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |