2A3K
Crystal Structure of the Human Protein Tyrosine Phosphatase, PTPN7 (HePTP, Hematopoietic Protein Tyrosine Phosphatase)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9207 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.125, 80.968, 100.392 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.680 - 2.550 |
R-factor | 0.22184 |
Rwork | 0.219 |
R-free | 0.27466 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jln |
RMSD bond length | 0.011 |
RMSD bond angle | 1.270 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.128 | 0.480 |
Number of reflections | 10833 | |
Completeness [%] | 99.4 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl, 2.0M ammonium dihydrogen phosphate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |