2A2K

Crystal Structure of an active site mutant, C473S, of Cdc25B Phosphatase Catalytic Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Collection date	2004-08-16
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	50.247, 70.933, 74.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.680 - 1.520
R-factor	0.182
Rwork	0.182
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ymk
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	23.680
High resolution limit [Å]	1.520
Number of reflections	37967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	DTT, Ammonium Sulfate, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K