2A2F

Crystal Structure of Sec15 C-terminal domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	298
Detector technology	CCD
Collection date	2003-12-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97958,0.97949,0.96308
Spacegroup name	C 2 2 21
Unit cell lengths	80.500, 93.900, 123.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.500
Rwork	0.243
R-free	0.27600
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.260
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	15141
Completeness [%]	91.5	80.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	sodium Citrate, ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	sodium Citrate, ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	sodium Citrate, ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K