2A2E

Crystal structure of the RNA subunit of Ribonuclease P. Bacterial A-type.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-11
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	179.277, 85.231, 101.912
Unit cell angles	90.00, 96.83, 90.00

Refinement procedure

Resolution	8.000 - 3.850
R-factor	0.356
Rwork	0.344
R-free	0.36170
Starting model (for MR)	1u9s
RMSD bond length	0.009
RMSD bond angle	1.870
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.050	3.950
High resolution limit [Å]	3.850	3.850
Rmerge	0.060	0.231
Number of reflections	13927
<I/σ(I)>	11.5	2.8
Completeness [%]	97.2	98.9
Redundancy	2.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	303	25% MPD, 200 mM KCl, 5 mM SrCl2, 50 mM MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 303K
1	VAPOR DIFFUSION, SITTING DROP	6.5	303	25% MPD, 200 mM KCl, 5 mM SrCl2, 50 mM MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 303K
1	VAPOR DIFFUSION, SITTING DROP	6.5	303	25% MPD, 200 mM KCl, 5 mM SrCl2, 50 mM MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 303K
1	VAPOR DIFFUSION, SITTING DROP	6.5	303	25% MPD, 200 mM KCl, 5 mM SrCl2, 50 mM MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 303K