2A1J
Crystal Structure of the Complex between the C-Terminal Domains of Human XPF and ERCC1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 116 |
| Detector technology | CCD |
| Collection date | 2004-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00696, 1.00930 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 69.848, 69.848, 104.109 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.710 - 2.700 |
| R-factor | 0.24073 |
| Rwork | 0.239 |
| R-free | 0.27483 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.123 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 8442 | |
| Completeness [%] | 99.9 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | Sodium Citrate, Ammonium Sulfate, Sodium Chloride, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
