2A11
Crystal Structure of Nuclease Domain of Ribonuclase III from Mycobacterium Tuberculosis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793, 1.000, 1.100 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.589, 72.589, 96.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.286 |
Rwork | 0.259 |
R-free | 0.28570 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.049 | 0.252 |
Number of reflections | 15508 | |
<I/σ(I)> | 28 | 5 |
Completeness [%] | 99.1 | 99.6 |
Redundancy | 4.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 15% PEG 3000, 100 mM Ca-acetate, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 15% PEG 3000, 100 mM Ca-acetate, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 15% PEG 3000, 100 mM Ca-acetate, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |