2A0I
F Factor TraI Relaxase Domain bound to F oriT Single-stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 62 |
| Unit cell lengths | 77.515, 77.515, 114.369 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.870 - 2.720 |
| R-factor | 0.221 |
| Rwork | 0.215 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1P4D Chain A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.820 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.048 | 0.331 |
| Number of reflections | 9891 | |
| Completeness [%] | 87.1 | 62.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 1000, Imidazole, Calcium Chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 1000 | ||
| 2 | 1 | 1 | Imidazole | ||
| 3 | 1 | 1 | Calcium Chloride | ||
| 4 | 1 | 2 | PEG 1000 | ||
| 5 | 1 | 2 | Imidazole | ||
| 6 | 1 | 2 | Calcium Chloride |
