29VF
RNA polymerase II initially transcribing complex with a 2-nt RNA and the +1 nucleosome
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.5 |
| Software | Warp,RELION,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
