29VD
Crystal structure of the human METTL3-METTL14 in complex with STC-15
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.248, 64.248, 226.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.040 - 2.350 |
| R-factor | 0.2772 |
| Rwork | 0.274 |
| R-free | 0.33730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.203 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.600 | 2.386 |
| High resolution limit [Å] | 2.345 | 2.345 |
| Number of reflections | 23737 | 1189 |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 16.7 | |
| CC(1/2) | 0.981 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20% PEG 3350, 0.4M Magnesium acetate |
