29TQ

Three viral endonucleases bound to the same inhibitor (9-hydroxy-3,4-dihydro-2H-pyrazino[1,2-c]pyrimidine-1,8-dione derivative). (3) Lassa virus L protein endonuclease with manganese ions.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-25
Detector	MAR CCD 300 mm
Wavelength(s)	0.8726
Spacegroup name	P 43 21 2
Unit cell lengths	38.680, 38.680, 254.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.240 - 2.700
R-factor	0.2181
Rwork	0.216
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.475
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (2.0_5936)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.200	0.732
Rmeas	0.237	0.866
Number of reflections	10084	1057
<I/σ(I)>	7.16	2.11
Completeness [%]	99.6	99.5
Redundancy	3.46
CC(1/2)	0.974	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	288	0.191 M Na citrate 0.1M Hepes pH7 4% PEG3350 0.1 M SrCl2 Cryo: 20% glycerol