29MP
Crystal structure of human CLK3 in complex with RNAZ88
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.933, 57.277, 73.783 |
| Unit cell angles | 93.81, 103.74, 110.64 |
Refinement procedure
| Resolution | 52.920 - 2.500 |
| R-factor | 0.22414 |
| Rwork | 0.222 |
| R-free | 0.27228 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.437 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.920 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 22505 | 2503 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.991 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 20% PEG3350 0.1M bis-tris propane 0.2 Ammonium Sulfate |
