29JC
Crystal structure of Coxsackievirus A16 (G-10) 2A protease determined via sulfur phasing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 50 |
| Detector technology | PIXEL |
| Collection date | 2026-01-21 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.7552 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.028, 56.773, 64.432 |
| Unit cell angles | 90.00, 94.95, 90.00 |
Refinement procedure
| Resolution | 64.190 - 1.801 |
| R-factor | 0.1624 |
| Rwork | 0.161 |
| R-free | 0.18710 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.910 |
| Data reduction software | xia2.multiplex |
| Data scaling software | DIALS (3.26.0.2) |
| Phasing software | CRANK2 |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.190 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.188 |
| Rmeas | 0.073 | 0.218 |
| Rpim | 0.017 | 0.107 |
| Number of reflections | 28519 | 4394 |
| <I/σ(I)> | 31 | 4.6 |
| Completeness [%] | 99.1 | 86.98 |
| Redundancy | 14.5 | 3.5 |
| CC(1/2) | 0.999 | 0.941 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293.15 | 0.1 M MES, pH 6.7, 13%-19% w/v PEG 20,000 |
