29IX
Human monosomes with bound toxin NT-2, focus on 60S subunit. NT-2 added to purified ribosomes as control.
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.2 |
| Software | cryoSPARC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
