28ZU

human 48S PIC with Kozak mRNA and eIF1A W70A mutant

This is a non-PDB format compatible entry.

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	TFS FALCON 4i (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.2
Software	cryoSPARC,RELION,PHENIX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION