28YR

ALFA-tag-FtnA from E.Coli isolated from in vitro translation reaction

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	TFS Selectris X
Detector	TFS FALCON 4i (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.04
Number of Particle	3407631
Software	cryoSPARC,PHENIX,Coot,EPU,UCSF ChimeraX
Symmetry	O
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION