28WB
Crystal structure of the STRUBBELIG-RECEPTOR FAMILY 6 (SRF6) ectodomain from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-26 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.977944 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.720, 76.720, 86.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.948 - 1.501 |
| Rwork | 0.162 |
| R-free | 0.19500 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.798 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.950 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.174 | 2.580 |
| Number of reflections | 39355 | 6330 |
| <I/σ(I)> | 13.9 | 1.11 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 13.6 | 12.9 |
| CC(1/2) | 0.999 | 0.508 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 25% PEG3350, 0.2M NaCl, 0.1M citrate pH 4.0 |
