28VW

Human galectin 3 apo in ammonium formate buffer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-02-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	36.471, 57.658, 62.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.470 - 1.090
Rwork	0.133
R-free	0.15250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.391
Data reduction software	DIALS (3.23.0-g7aff524e7-release)
Data scaling software	Aimless (0.8.2)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.126))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.470	31.470	1.110
High resolution limit [Å]	1.090	5.970	1.090
Rmerge	0.107	0.077	0.855
Rmeas	0.122	0.090	1.065
Rpim	0.059	0.045	0.624
Number of reflections	54684	406	2320
<I/σ(I)>	10.6	20.7	3
Completeness [%]	98.6	99.4	86.3
Redundancy	7.9	6.7	4.9
CC(1/2)	0.989	0.990	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20%Peg 3350, 200mM ammonium formate