28VP

GAL-3 protein with lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-02-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	36.999, 58.184, 63.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.810 - 1.520
Rwork	0.209
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0431 (REFMACAT 0.4.126))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.810	42.810	1.550
High resolution limit [Å]	1.520	8.330	1.520
Rmerge	0.248	0.148	1.724
Rmeas	0.276	0.163	1.921
Rpim	0.119	0.067	0.839
Number of reflections	21717	173	1084
<I/σ(I)>	4.8	9.1	1.2
Completeness [%]	100.0	99.8	100
Redundancy	9.9	7.8	10.3
CC(1/2)	0.988	0.989	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Ammonium iodide 20% w/v PEG 3350