28UN

E. coli 70S ribosome, conformational excited state mutation in SSU-h41, with A/P- and P/E-site tRNA

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	GATAN K3 BIOQUANTUM (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.48
Software	cryoSPARC,EPU,UCSF ChimeraX,REFMAC
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION