28UA

LASV cap-snatching endonuclease, serial synchrotron apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P09 HiPhaX
Synchrotron site	PETRA III, DESY
Beamline	P09 HiPhaX
Temperature [K]	293
Detector technology	PIXEL
Collection date	2024-12-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.7749
Spacegroup name	P 43 21 2
Unit cell lengths	58.630, 58.630, 135.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.460 - 1.810
R-factor	0.1852
Rwork	0.182
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.706
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.21.2-5419_9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.210	1.860
High resolution limit [Å]	1.810	1.810
Number of reflections	22831	1736
<I/σ(I)>	7
Completeness [%]	100.0
Redundancy	99.7
CC(1/2)	0.989	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	After limited proteolysis with subtilisin A, 7.5 mg/ml target protein were mixed with an equal volume of crystallization solution (10% w/v PEG 10000, 250 mM MgSO4 and 100 mM Tris/HCl pH 9.0)