28SV
Structure of ASBT homologue from Leptospira biflexa in inward-facing form (Crystal Form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.583, 94.902, 107.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.000 - 2.210 |
| R-factor | 0.1806 |
| Rwork | 0.178 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.316 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 2.280 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.240 | 1.621 |
| Rmeas | 1.804 | |
| Rpim | 0.107 | 0.784 |
| Number of reflections | 34241 | 2770 |
| <I/σ(I)> | 6.13 | 0.94 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 6.2 | 5.3 |
| CC(1/2) | 0.466 | 0.403 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8.5 | 293 | 0.12M Magnesium formate dihydrate, 0.1M Sodium chloride, 0.1M Tris pH 8.5, 33% PEG 600 |
