28RM
Crystal structure of the human galectin-7 in complex with p-Methoxyphenyl 3-O-toluensulfonyl-beta-D- galactopyranoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.224, 53.270, 72.320 |
| Unit cell angles | 90.00, 94.22, 90.00 |
Refinement procedure
| Resolution | 29.880 - 1.900 |
| R-factor | 0.23477 |
| Rwork | 0.233 |
| R-free | 0.27437 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 38138 | 3744 |
| <I/σ(I)> | 9.03 | |
| Completeness [%] | 97.0 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.995 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM Bis-Tris propane, pH 7.0, 13% PEG 3350 |
