28PB
Crystal structure of CbcA periplasmic domain from Geobacter sulfurreducens
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-12 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.436, 94.167, 114.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.752 - 1.910 |
| Rwork | 0.199 |
| R-free | 0.25310 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.177 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.105)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.170 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.199 | |
| Rmeas | 0.207 | |
| Rpim | 0.056 | |
| Number of reflections | 58831 | 2906 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.7 | 14.2 |
| CC(1/2) | 0.991 | 0.308 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 1.4 M trisodium citrate and 0.1 M sodium HEPES (pH 8) |
