28NV
Human Adenovirus type C5 knob protein with the KO1 mutation and A20 insertion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 85.283, 85.283, 85.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.300 - 1.270 |
| R-factor | 0.1573 |
| Rwork | 0.157 |
| R-free | 0.17112 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.634 |
| Data reduction software | XDS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.300 | 1.290 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.083 | 2.240 |
| Rmeas | 0.084 | 2.386 |
| Rpim | 0.014 | 0.789 |
| Number of reflections | 54652 | 2700 |
| <I/σ(I)> | 22.9 | 0.4 |
| Completeness [%] | 100.0 | 99.2 |
| Redundancy | 32.8 | 8.6 |
| CC(1/2) | 1.000 | 0.353 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.2 M Sodium malonate dibasic monohydrate, 0.1 M Bis-Tris propane, 20 % w/v PEG 3350 |
