28NB
crystal structure of Dpo31, of a tail-spike protein with depolymerase activity identified in a marine podovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-05 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9801112 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.744, 69.744, 533.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.330 - 3.300 |
| R-factor | 0.25 |
| Rwork | 0.247 |
| R-free | 0.25930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 3.530 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.520 | 1.140 |
| Rpim | 0.137 | 0.329 |
| Number of reflections | 24378 | 3654 |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.9 | 12.9 |
| CC(1/2) | 0.917 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 27% PEG 3350, 21% isopropanol, 100 mM Tris HCl pH 8.5 |
