28MG
crystal structure of Dpo31, of a tail-spike protein with depolymerase activity identified in a marine podovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.727, 75.607, 124.937 |
| Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
| Resolution | 47.380 - 2.150 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (21-NOV-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.115 | 0.543 |
| Rpim | 0.050 | 0.351 |
| Number of reflections | 59091 | 1200 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 63.4 | 6.7 |
| Redundancy | 6.2 | 2.3 |
| CC(1/2) | 0.997 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 283 | 20% PEG 3350 and 200 mM Potassium nitrate. drop ratio 2:1 (protein to reservoir) |
