28LZ
Iron loaded human H-chain ferritin, 20 minute oxygen soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.760, 182.760, 182.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.380 - 1.940 |
| R-factor | 0.1691 |
| Rwork | 0.168 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.979 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.380 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 37937 | 2100 |
| <I/σ(I)> | 39.05 | |
| Completeness [%] | 96.4 | |
| Redundancy | 2 | |
| CC(1/2) | 0.998 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | 0.1 M bicine pH 9.0 0.1 M sodium chloride 2.0 M magnesium chloride 60 mM ferrous chloride 3 mM sodium azide |
