28LX

crystal structure of Dpo31, of a tail-spike protein with depolymerase activity identified in a marine podovirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97911
Spacegroup name	H 3 2
Unit cell lengths	88.200, 88.200, 643.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.200 - 2.200
R-factor	0.253
Rwork	0.252
R-free	0.27300
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.830
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (21-NOV-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.115	0.875
Rpim	0.026	0.295
Number of reflections	22469	300
<I/σ(I)>	18.4
Completeness [%]	43.7	5.7
Redundancy	21.2	8.9
CC(1/2)	1.000	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	Hanging drops, 2 microL of protein (12 mg/ml) with 1 microL of reservoir solution 1.2 M Ammonium sulfate and 100 mM Citric acid at pH 5.0