28LK

Crystal structure of complement-inhibiting protein ChiB of Borrelia recurrentis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	82.010, 82.010, 90.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.680 - 1.500
R-factor	0.1839
Rwork	0.181
R-free	0.21500
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.201
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.680	1.555
High resolution limit [Å]	1.500	1.501
Number of reflections	49770	4840
<I/σ(I)>	13.6
Completeness [%]	99.8
Redundancy	10.6
CC(1/2)	0.999	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1.7 M Amoniumcitrat, 5mM DTT