28JI

LASV cap-snatching endonuclease, cryo apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.03322
Spacegroup name	P 43 21 2
Unit cell lengths	57.687, 57.687, 135.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.080 - 1.610
R-factor	0.2252
Rwork	0.224
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.864
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2-5419_9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.080	1.640
High resolution limit [Å]	1.610	1.610
Rmeas	0.102
Number of reflections	30633	1501
<I/σ(I)>	16.9	1.3
Completeness [%]	100.0	100
Redundancy	26.5
CC(1/2)	0.999	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	After limited proteolysis with subtilisin A, 7.5 mg/ml protein were mixed with an equal volume of crystallization solution (10% w/v PEG 10000, 250 mM MgSO4 and 100 mM Tris/HCl pH 9.0)