28JF
BKPyV VP1 IN COMPLEX WITH VHH016
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.580, 135.270, 167.830 |
| Unit cell angles | 90.00, 97.79, 90.00 |
Refinement procedure
| Resolution | 116.767 - 2.593 |
| Rwork | 0.211 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.306 |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.105)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.767 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rpim | 0.004 | |
| Number of reflections | 160823 | 5239 |
| <I/σ(I)> | 5.1 | 0.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.3 | 5.9 |
| CC(1/2) | 0.980 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293.15 | 30% (v/v) PEG 400, 100 mM Sodium acetate/Acetic acid pH 4.5, 200 mM Calcium acetate |
