28BR

Cryogenic temperature crystal structure of Nmar_1308 protein at 3.00 angstrom resolution

Replaces:  9UL9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	75.480, 75.480, 208.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.650 - 3.000
R-factor	0.1957
Rwork	0.192
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.369
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.650	3.180
High resolution limit [Å]	3.000	3.000
Number of reflections	26056	4151
<I/σ(I)>	8.35
Completeness [%]	97.6
Redundancy	5.98
CC(1/2)	0.980	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	0.3M Sodium nitrate, 0.3 Sodium phosphate dibasic, 0.3M Ammonium sulfate, 1.0 M Tris (base), BICINE pH 8.5, 25% v/v MPD, 25% PEG 1000, 25% w/v PEG 3350