28JB

Structure of DarM methyltransferase in complex with SAH

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.8731
Spacegroup name	P 21 21 21
Unit cell lengths	38.722, 67.502, 91.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.390 - 1.650
Rwork	0.176
R-free	0.19150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.092
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1-5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.860	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.058	0.898
Rmeas	0.063	0.995
Rpim	0.024	0.419
Number of reflections	29799	1451
<I/σ(I)>	21.7	2.3
Completeness [%]	100.0	100
Redundancy	13	10.4
CC(1/2)	1.000	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M magnesium chloride, 0.1 M Tris-HCl pH 8.5 and 30% (w/v) PEG4000