28HT
Crystal structure of the Ubiquitin Conjugating Enzyme 4 from Leishmania major (LmUbC4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99990 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 115.000, 115.000, 151.542 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.230 - 2.160 |
| R-factor | 0.19243 |
| Rwork | 0.191 |
| R-free | 0.22424 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.295 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.230 | 2.194 |
| High resolution limit [Å] | 2.157 | 2.157 |
| Number of reflections | 20142 | 1051 |
| <I/σ(I)> | 20.1 | 0.9 |
| Completeness [%] | 95.9 | 100 |
| Redundancy | 18.9 | |
| CC(1/2) | 0.998 | 0.357 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 3 M NaCl, 0.1 M MES/imidazole pH 6.5, 20% glycerol |
