285D
X-RAY AND SOLUTION STUDIES OF DNA OLIGOMERS AND IMPLICATIONS FOR THE STRUCTURAL BASIS OF A-TRACT-DEPENDENT CURVATURE
Experimental procedure
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1991-06-01 |
Detector | XENTRONICS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.400, 40.215, 65.268 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.206 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | B-DNA ORIENTED FIBER |
RMSD bond length | 0.033 |
RMSD bond angle | 0.078 |
Data reduction software | XENGEN |
Data scaling software | ULTIMA |
Refinement software | NUCLSQ |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.500 | 2.200 * |
Rmerge | 0.084 | |
Number of reflections | 2152 | |
Completeness [%] | 87.5 | 60 * |
Redundancy | 4.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SPERMINE_HCL | ||
5 | 1 | 1 | NA CACODYLATE | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 2 (mg/ml) | |
2 | 1 | drop | 12.5 (mM) | ||
3 | 1 | drop | spermine-4HCl | 1.2 (mM) | |
4 | 1 | drop | sodium cacodylate | 20 (mM) | |
5 | 1 | drop | MPD | 10 (%(w/v)) | |
6 | 1 | reservoir | MPD | 30 (%(w/v)) |