284D
THE BI-LOOP, A NEW GENERAL FOUR-STRANDED DNA MOTIF
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 22.844, 27.676, 55.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.000 - 1.100 |
| R-factor | 0.183 * |
| Structure solution method | HEAVY ATOM METHOD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.006 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-96 |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.100 |
| Rmerge | 0.067 * |
| Number of reflections | 19971 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | BA ACETATE | ||
| 4 | 1 | 1 | PEG 4000 | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cacodylate | 8.8 (mM) | |
| 2 | 1 | drop | barium acetate | 13.2 (mM) | |
| 3 | 1 | drop | PEG4000 | 14.7 (%(w/v)) | |
| 4 | 1 | reservoir | PEG4000 | 30 (%(w/v)) |
