27PT
Dictyostelium discoideum cytoplasmic dynein motor domain in complex with ADP.Vi (Phi-particle)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.2 |
| Number of Particle | 1755826 |
| Software | cryoSPARC,PHENIX,SerialEM,UCSF ChimeraX,Coot |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
