27JO

Crystal structure of the mature form of human ASPRV1 bound to indinavir

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2026-04-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97853
Spacegroup name	P 6
Unit cell lengths	105.934, 105.934, 55.128
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.900 - 2.080
R-factor	0.1997
Rwork	0.197
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.866
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.900	2.130
High resolution limit [Å]	2.080	2.080
Number of reflections	21380	734
<I/σ(I)>	22.5
Completeness [%]	100.0
Redundancy	20.1
CC(1/2)	0.990	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.05 M calcium chloride dihydrate, 0.1 M MES monohydrate, pH 6.0, and 45% (v/v) PEG 200