27AA
Crystal structure of the complex of short peptidoglycan recognition protein from Camelus dromedarius with nonanoic acid at 1.83 A resolution
Replaces: 5DWFExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 88.775, 101.525, 163.087 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.256 - 1.834 |
| Rwork | 0.272 |
| R-free | 0.32110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.436 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 64628 | 1288 |
| <I/σ(I)> | 2 | 2 |
| Completeness [%] | 99.8 | |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 3350 Sodium potassium tartarate |
