27WI

UBE2D2 bound with compound XY54

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-02
Detector	RIGAKU HyPix-Arc 150
Wavelength(s)	1.54184
Spacegroup name	P 21 21 21
Unit cell lengths	43.275, 43.971, 78.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.680 - 2.000
R-factor	0.1812
Rwork	0.175
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.991
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.680	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.052	0.168
Number of reflections	10623	6539
<I/σ(I)>	34.1
Completeness [%]	99.7
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.1 M sodium chloride, 0.1 M BICINE (pH 9.0), and 20% (v/v) polyethylene glycol monomethyl ether 550