27IQ

Crystal structure of the mature form of human ASPRV1 bound to filaggrin peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2026-01-13
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	51.004, 64.929, 76.221
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.740 - 2.090
R-factor	0.2646
Rwork	0.262
R-free	0.29220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.069
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.600	2.130
High resolution limit [Å]	2.090	2.090
Number of reflections	28780	1419
<I/σ(I)>	11.6
Completeness [%]	97.7
Redundancy	9.8
CC(1/2)	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M calcium chloride dihydrate, 0.1 M sodium acetate trihydrate, pH 4.6, and 30% (w/v) PEG 4000