26LN
Crystal structure of a new isoform of ribosome inactivating protein from Momordica balsamina at 1.41 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-28 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | H 3 |
| Unit cell lengths | 130.303, 130.303, 39.853 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.644 - 1.408 |
| Rwork | 0.179 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mrw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.894 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.644 | 1.430 |
| High resolution limit [Å] | 1.408 | 1.410 |
| Rmerge | 0.034 | 1.284 |
| Rmeas | 0.042 | |
| Rpim | 0.025 | |
| Number of reflections | 48750 | 2502 |
| <I/σ(I)> | 18.1 | 1 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 5.2 | 5.4 |
| CC(1/2) | 0.999 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | PEG 6000, 0.1M Sodium Phosphate |
