26AP

Complex between N-lobe Arc mutant F267/F5Phe and nanobody H11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX3
Synchrotron site	Australian Synchrotron
Beamline	MX3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-02-13
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	40.838, 48.370, 43.709
Unit cell angles	90.00, 96.57, 90.00

Refinement procedure

Resolution	28.080 - 1.500
R-factor	0.1619
Rwork	0.160
R-free	0.19360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.029
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.080	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.084
Number of reflections	26954	2645
<I/σ(I)>	12
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	0.999	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291.15	30% PEG 3350, 0.2 M magnesium chloride, 0.1 M TRIS hydrochloride pH 8.0