25YR
High-resolution crystal structure of Arp2/3 complex inhibitor Arpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 65 |
| Unit cell lengths | 80.335, 80.335, 124.356 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.360 - 1.650 |
| R-factor | 0.1699 |
| Rwork | 0.169 |
| R-free | 0.19380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.360 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.099 | 0.126 |
| Number of reflections | 54508 | 2747 |
| <I/σ(I)> | 7.2 | 6.05 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.6 M Sodium citrate, pH 6.5. Protein was in 50 mM Tris-HCl pH 8.0, 200 mM NaCl at a concentration of 10 mg/mL. |
