25EC

Crystal Structure of a Human Topoisomerase II Beta-DNA Cleavage Complex Trapped in N-gate Dissociated State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 32 2 1
Unit cell lengths	92.312, 92.312, 232.272
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.170 - 2.900
R-factor	0.2907
Rwork	0.289
R-free	0.32900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.532
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.170	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	26078	2532
<I/σ(I)>	19.72
Completeness [%]	99.4	98.52
Redundancy	17.76
CC(1/2)	0.996	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	50mM 2-(N-morpholino) ethanesulfonic acid, pH 5.5, 24% 2-methyl-2, 4-pentanediol (MPT), 0.1M magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K