254D
ALTERNATING AND NON-ALTERNATING DG-DC HEXANUCLEOTIDES CRYSTALLIZE AS CANONICAL A-DNA
Experimental procedure
Source type | ROTATING ANODE |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1992-01-01 |
Detector | RIGAKU |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 39.670, 45.980, 39.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.176 * |
Rwork | 0.197 |
R-free | 0.18600 |
RMSD bond length | 0.013 * |
RMSD bond angle | 3.500 * |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 2937 |
Completeness [%] | 68.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | SPERMINE | ||
5 | 1 | 1 | MGCL2 | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 1 (mM) | |
2 | 1 | drop | sodium cacodylate | 15 (mM) | |
3 | 1 | drop | spermine | 0.5-5 (mM) | |
4 | 1 | drop | 1-20 (mM) | ||
5 | 1 | drop | MPD | 5 (%(v/v)) | |
6 | 1 | reservoir | MPD | 35 (%) |